Effects of RNAi Silencing GTPBP4 Gene on Proliferation and Apoptosis of Colon Cancer RKO Cells

Objective To investigate the changes of RKO cell proliferation and apoptosis after GTPBP4 gene silencing.

Methods GTPBP4-siRNA and negative control CON053 were successfully transfected into colon cancer RKO cell line. Real-time PCR and Western blot were used to detect the knock down efficiency. Cellomics cell count was used to detect cell growth, MTT method was used to detect cell proliferation, FACS method was used to detect cell cycle, and cell clone formation experiment was carried out, cell apoptosis was analysed by AnnexinⅤ-APC single staining flow cytometry.

Results Slow virus successfully infected RKO cells, mRNA and protein detection showed that the GTPBP4 gene was knocked down successfully. After GTPBP4 gene knocked down, the cell proliferation rate significantly inhibited, MTT value ratio (i.e., multiplication) decreased, G_0/1 and G₂/M phase cells increased, S phase decreased, cell colony number decreased, apoptosis peak value was significantly higher than that in the control group, and the peak value appears earlier than that of the control group.

Conclusion GTPBP4 gene may affect the occurrence and development of colon cancer by promoting tumor cell proliferation and inhibiting apoptosis.