Effect of Jinlong Capsule on Vasculogenic Mimicry and Mig-7 of Colon Cancer

Objective To explore the effect of Jinlong capsule on vasculogenic mimicry and Mig-7 of colon cancer.

Methods To observe the capacity of forming VM about HCT116 colon cancer cells and HT29 colon cancer cells when cultured in three dimensional condition. The VM (+) cells for subsequent experiments, which were divided into blank group, Chinese medicine group (Jinlong capsule) and positive control group (5-fluorouracil, 200μmol/L). The Jinlong capsule culture was divided into low dose group (0.01mg/ml), medium dose group (0.02mg/ml) and high dose group (0.04mg/ml). The expression of Mig-7 mRNA and protein were tested by RT-PCR and Western blot, respectively.

Results HCT116 colon cancer cells could form visible VM structure and express Mig-7. The high, medium, and low dose Jinlong Capsule and 5-fluorouracil group could inhibit the capacity of forming VM about HCT116 colon cancer cells and reduce the expression of Mig-7 mRNA and protein. The expression of VM and Mig-7 of each treated group were lower than that in the blank control group, the difference was statistically significant(P<0.05). The VM and Mig-7 expression gradually reduced with the increase of Jinlong capsule dose. The effect of Jinlong capsule high dose group (0.04 mg/ml) and 5-fluorouracil group were superior to others(P<0.01), and 5-fluorouracil group was better than Jinlong capsule high-dose group (0.04mg/ml) (P<0.05).

Conclusion The VM(+) colon cancer cells could express Mig-7 mRNA and protein. Jinlong capsule can inhibit the formation of VM probably by reducing the expression of Mig-7 mRNA and protein.