| Citation: |
LIU Runtian, AN Congjing, BAI Yun, ZHENG Jianxing. Effects of GSTP1 on Proliferation and Invasion of Human HepG2 Cells[J]. Cancer Research on Prevention and Treatment, 2016, 43(12): 1039-1042. DOI: 10.3971/j.issn.1000-8578.2016.12.006
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To investigate the effect of GSPT1 overexpression or knockdown on the proliferation and invasion of human HepG2 cells.
HepG2 cells were infected with adenovirus (Ad)-GSTP- or Ad-shGSTP1 to overexpress or knockdown the expression of GSTP1 in HepG2 cells. The expression level of GSTP1 mRNA was examined by quantitative PCR; CCK-8 and Transwell assay were used to determine the cell viability and invasion, respectively. The expression of Akt, mTOR, p-Akt and p-mTOR were detected by Western blot.
The mRNA expression of GSTP1 was up-regulated after infection with Ad-GSTP1, and was down-regulated after infection with Ad-shGSTP1(P<0.05). The overexpression of GSTP1 significantly inhibited the viability and invasion of HepG2 cells(P<0.05), however, the knockdown of GSTP1 enhanced cell viability and invasion(P<0.05). Moreover, the phosphorylation level of Akt and mTOR were increased in the cells infected with Ad-GSTP1(P<0.05). Inverse results were obtained in the cells infected with Ad-shGSTP1.
The viability and invasion of HepG2 cells could be inhibited by GSTP1 overexpression while enhanced by GSTP1 knockdown, which may be related with Akt/mTOR pathway.
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