Effect of SAHA on Proliferation and Apoptosis of Breast Cancer Cells MDA-MB-435 and Related Mechanism

Objective To investigate the effects of SAHA on the proliferation and apoptosis of human breast cancer cells MDA-MB-435 and related mechanisms.

Methods After the treatment with different concentrations of SAHA, the proliferation, apoptosis, cell cycle arrest and protein expression of MDA-MB-435 cells were detected by MTT, FCM, Western blot and RT-PCR, respectively.

Results MTT results showed that SAHA could inhibit cells proliferation in a dose-dependent manner, and FCM analysis showed that SAHA could induce ₂/M cell cycle arrest, downregulate mitochondria membrane potential, produce ROS and induce early apoptosis. Moreover, SAHA could downregulate cell cycle protein cyclin B, activate p38-MAPK and JNK, and inhibit P53 expression, and may not be via PI3K, P38 MAPK, ERK1/2, NF-κB or JNK pathways.

Conclusion SAHA could inhibit the proliferation, induce cell cycle arrest and early apoptosis, and activate relative cell proliferation molecules in MDA-MB-435 cells, which suggesting that SAHA could be the drug candidate to breast cancer.