miR-216b Inhibits Cervical Cancer Cells Autophagy by Regulating Beclin-1 Expression

Objective To investigate the effect of miR-216b on autophagy in cervical cancer HeLa cells.

Methods After the transfection of miR-216b and the application of their inhibitors,we used GFP-LC3 shRNA transfection to test the autophagy levels of HeLa cells and Western blot to test the expression changes of autophagy-related genes Beclin-1 and LC3-Ⅱ.

Results After transfected with miR-216b,autophagy related gene Beclin-1 was inhibited in HeLa cells,the expression of LC3-Ⅱ was increased,and autophagy was inhibited; however,the level of autophagy was increased when the expression of miR-216b was inhibited. Further,miR-216b can inhibit the occurrence of autophagy by inhibiting the expression of Beclin-1 by targeting Beclin-1 3’UTR.

Conclusion  miR-216b could inhibit HeLa cells autophagy,which provides a theoretical basis for the clinical treatment of cervical cancer.