Effect of RORα Overexpression on Proliferation, Migration and Invasion of Human Gastric MGC803 Cells

Objective To investigate the effect of RORα overexpression on human gastric cancer MGC803 cells proliferation,migration and invasion.

Methods The expressions of RORα,MMP-9 and TIMP3 mRNA and protein were detected by Real-time PCR or RT-PCR and Western blot. The effect of RORα overexpression on the proliferation,cell cycle,migration and invasion of MGC803 cells were detected by MTT,flow cytometry,wound healing and Transwell assays.

Results The expressions of RORα mRNA and protein were stably increased in the RORα/MGC803 cells. The proliferation activity in RORα/MGC803 cells were notably lower than in MGC803 cells and in vector/MGC803,respectively,at 48 h,72 h and 96 h(P＜0.05) . Percentage of G₂/M in RORα/MGC803 cells markedly higher than that in MGC803 cells and vector/MGC803(P＜0.05) . The migration length in RORα/MGC803 cells was markedly lower than that in MGC803 and vector/MGC803 cells (P＜0.05) . The cells through the matrigel coated membrane in RORα/MGC803 significantly decreased comparing with MGC803 and vector/MGC803 cells(P＜0.05) . RORα overexpression was downregulation of MMP-9 and upregulation of TIMP3 mRNA and proteins(P＜0.05) . RORα overexpression could significantly downregulate MMP-9 and upregulate TIMP3 mRNA and proteins(P＜0.05) .

Conclusion  RORα/MGC803 cells with stably overexpressed RORα are successfully constructed. The overexpression of RORα may inhibit the proliferation,migration and invasion,as well as G₂/M arrest in MGC803 cells,which may be correlated with the downregulation of MMP-9 and the upregulation of TIMP3.