Effect of RNA Interference Decreasing MSI1 Expression on Chemosensitivity of Human Glioma Cells

Objective  To explore the effect of RNA interference decreasing MSI1 gene expression on the chemosensitivity of human glioma cells U-87MG.

Methods  The design and synthesis of Msi1-specific siRNA and the transfection of U-87MG cells were conducted. The expression of MSI1 in U-87MG cells was detected by Western blot. CCK-8 was employed to observe the sensitivity of U-87MG cells to temozolomide(TMZ). FCM was used to observe the apoptosis rate of U-87MG cells after pSIREN-MSI1 transfection.

Results  Lentiviral vector-mediated MSI1-siRNA was successfully constructed. Immunofluorescence assay demonstrated that the transfection efficiency was above 60%. Western blot analyses demonstrated that pSIREN-MSI1 could significantly inhibit the expression of MSI1 in U-87MG cells (P<0.01); CCK-8 results showed that when U-87MG cells were exposed to temozolomide, the growth inhibiting rate of pSIREN-MSI1 transfected cells was significantly increased, compared with those of blank control group and negative control group (P<0.01). FCM results showed that the apoptosis rate of U-87MG cells were increased after pSIREN-MSI1 transfection(P<0.01).

Conclusion  MSI1-siRNA could significantly inhibit the expression of MSI1 in human glioma cells U-87MG. It could induce the apoptosis and enhance the chemosensitivity of U-87MG cells.