Objective To detect the expression of ChREBP mRNA and its CpG island methylation in human hepatocellular carcinoma tissues and investigate their correlation.
Methods Bisulfite Genomic sequencing was used to analyze the methylation level of 29 CpG sites in CpG island of ChREBP in 45 paired hepatocellular carcinoma and adjacent non-tumor tissues. The expression of ChREBP mRNA was detected in 31 paired hepatocellular carcinoma and adjacent non-tumor tissues using quantitative real-time PCR, meanwhile, the relationship between the level of methylation status and mRNA expression was analyzed.
Results The methylation level of some CpG sites (5, 6, 7, 14) were significantly lower in hepatocellular carcinoma tissues than those in the adjacent non-tumor tissues (P<0.05). There was no statistical significancet difference in the methylation level of other CpG sites between hepatocellular carcinoma tissues and adjacent non-tumor tissues (all P<0.05). The methylation level of ChREBP at CpG 15, 18, 20, 23, 26 and 29 tended to be higher in patients with older age (≥50 years) than those in younger age(<50 years) (all P<0.05). The mRNA expression of ChREBP in hepatocellular carcinoma tissues was lower than that in adjacent non-tumor tissues (P=0.003), however, no significant relationship was observed between DNA methylation and the mRNA expression of ChREBP in hepatocellular carcinoma tissues(P<0.05).
Conclusion There was a down-regulated expression of ChREBP in hepatocellular carcinoma tissues, which could not be mediated by DNA methylation.
DownLoad: