Objective To upregulate neuropilin-1(NRP-1) expression in breast cancer cell lines MDA-MB-231 and SK-BR-3, and to investigate their effects on cells proliferation, apoptosis, invasion and migration.
Methods PcDNA3.1-NRP-1 expression vector was constructed and transfected into MDA-MB-231 and SK-BR-3 cell lines by liposome, then stably expressing cell lines were screened by different concentrations of G418. The expression levels of NRP-1 mRNA and protein were detected by RT-qPCR and Western blot. We used CCK-8 assay, AnnexinⅤ-APC/7-AAD assay, Transwell assay to detect the change of cells proliferation, apoptosis, invasion and migration.
Results pcDNA3.1-NRP-1 expression vector was successfully constructed and transfected into MDA-MB-231 and SK-BR-3 cell lines, the stably expressing cell lines were screened. NRP-1 expression was up-regulated significantly at both mRNA and protein levels, compared with control group(P<0.05). CCK-8 assay and Transwell assay showed that NRP-1 promoted the proliferation, invasion and migration of MDA-MB-231 and SK-BR-3 cell lines(all P<0.05). AnnexinⅤ-APC/7-AAD assay showed that NRP-1 inhibited the apoptosis of the two cell lines(P<0.05).
Conclusion NRP-1 plays a specific role in the development, invasion and migration of breast cancer. It can promote the proliferation, invasion and migration, and inhibit the apoptosis of breast cancer cells.
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