Inhibitory Effect of Thermosensitive Liposomes Combined with Microwave Thermotherapy on Human Cervical Carcinoma Cells HeLa

Objective  To construct the thermos-sensitive liposomes loading with doxorubicin drug delivery system (DOX-TSLs) and investigate the effect of its combination with microwave thermotherapy on human cervical carcinoma cells HeLa in vitro.

Methods  Reverse evaporation method was used to prepare DOXTSLs; drug loading rate and in vitro release were detected; MTT method was used to detect the inhibition of HeLa cells treated with chemotherapy, thermotherapy and the combination; the apoptosis rate was detected by flow cytometry.

Results  DOX-TSLs drug delivery system was constructed successfully with the average particles size (190.7±4.8) nm and the efficiency 88.5%; the in vitro release detection revealed that the cumulative release rate could reach 85% with microwave thermotherapy at 30 min; DOX could be delivered into HeLa cells within 6 h by DOX-TSLs; the combination therapy of DOX-TSLs and microwave thermotherapy could inhibit cell proliferation effectively and significantly promote cell apoptosis which could be reach (62.2±2.3) %.

Conclusion  DOX-TSLs combined with microwave thermotherapy in the treatment of human cervical carcinoma cells HeLa have a synergistic effect. This therapy provides newly theoretical and experimental basis of human cervical carcinoma.