Effect of Long Non-coding RNA LOC100294362 on Proliferation and Invasion of Breast Cancer Cells

Objective  To investigate the expression level of long non-coding RNA LOC100294362 in breast cancer tissues and cell lines, to observe the effect of LOC100294362 on the proliferation and invasion of breast cancer MCF-7 cells and to explore its possible mechanisms.

Methods  Quantitative reverse-transcription PCR(qRT-PCR) was performed to detect the relative expression of LOC100294362 in breast cancer tissues and cell lines. After MCF-7 cells were transfected with over-expressed or siRNA-LOC100294362, the effect of up-regulated or down-regulated LOC100294362 on MCF-7 cells proliferation and invasion were detected by MTT assay and Transwell assay, and the expression level of p53 protein was tested by Western blot assay.

Results  Compared with their corresponding normal tissues and cell lines, LOC100294362 was significantly highly expressed in breast cancer samples and cell lines. Over-expressed LOC100294362 enhanced MCF-7 cells proliferation and invasion abilities, and decreased the expression of p53 protein; however, silencing LOC100294362 inhibited MCF-7 cells proliferation and invasion abilities, and increased the expression of p53 protein.

Conclusion  LOC100294362 promotes MCF-7 cells proliferation and invasion probably by inhibiting the expression of p53 protein.