Effects of miR-711 on Invasion, Metastasis and Epithelial Mesenchymal Transition of Gastric Cancer Cells MGC-803

Objective To investigate the effects of miR-711 on the invasion, metastasis and epithelial mesenchymal transition (EMT) of human gastric cancer cells MGC-803. Methods miR-711 mimics, miR-711 inhibitors and miRNA empty plasmids were transfected into human gastric cancer cell strain MGC-803 by liposomes transient transfection method. miRNA empty plasmids transfection group was taken as the negative control group (NC group), and the blank transfection group taken as blank control group (K group). After 48h, the effectiveness of transfection was observed under fluorescence microscope. Cell scratch experiment and Transwell invasion experiment were used to detect the migration and invasion of transfected MGC-803 cells. Western blot was used to observe the expression of epithelial protein and mesenchymal protein of transfected MGC-803 cells. Results As revealed by Transwell invasion test: the number of invaded cells in NC and K groups were (120.00±4.58) and (119.67±5.13), while that was significantly lower in the miR-711 mimics transfection group (70.67±5.51) (both P<0.05); As revealed by the cell scratch test: at 48h after scratch, the healing rate of NC and K groups were (32.52±1.73)% and (32.15±1.55)%, while that was significantly lower of the miR-711 mimics transfection group(7.08±0.73)% (both P<0.05); As revealed by Western blot test: compared with NC and K groups, the epithelial markers (E-cadherin) relative protein expression was increased in the miR-711 mimics transfection group (both P<0.05); while the mesenchymal markers (Vimentin) expression was decreased(both P<0.05). Conclusion miR-711 overexpression could inhibit the invasion, metastasis and EMT of human gastric cancer cells MGC-803.