In vitro Investigation on Effect of Lentiviral Vector-mediated RNA Interference Inhibiting VASH1 Expression on Proliferation and Apoptosis of Human Glioma Cells U-87MG

Objective To investigate the effect of lentiviral vector-mediated RNA interference inhibiting VASH1 gene expression on the proliferation and apoptosis of human glioma cells U-87MG. Methods A lentiviral vector carrying short hairpinRNA(shRNA) of human VASH1 gene (pGCL-GFP-VASH1) was constructed and used to transfect 293T cells. The transfection efficiency was evaluated and then the lentiviral vector with suitable concentration was transfected into the human glioma cells U-87MG; RT-PCR and Western blot were used to detect the expression of VASH1 in U-87MG cells. MTT assay was used to observe the inhibion ratio of U-87MG cells growth. FCM analysis was used to observe the apoptosis. Results RT-PCR and Western blot analyses demonstrated that pGCL-GFP-VASH1 could significantly inhibit the expression of VASH1 mRNA and protein in U-87MG cells(P<0.01); MTT results showed that it could increase the growth of U-87MG cells(P<0.01). FCM results showed that the occurrence of apoptosis was suppressed significantly in U-87MG cells(P<0.01). Conclusion Lentiviral vector-mediated RNA interference targeting against VASH1 could effectively inhibit the expression of VASH1, induce the cell proliferation and suppress the apoptosis in U-87MG cells.