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GUO Jingjie, YUAN Changjing, LIU Li, LV Xiuwei, YU Tao, HE Wenyu, CHEN Yanhao. Effect of pCMV AIM-1 antisense Constructs on Human Ovarian Cancer Xenografts Growth in Nude Mice[J]. Cancer Research on Prevention and Treatment, 2015, 42(11): 1086-1090. DOI: 10.3971/j.issn.1000-8578.2015.11.006
Citation: GUO Jingjie, YUAN Changjing, LIU Li, LV Xiuwei, YU Tao, HE Wenyu, CHEN Yanhao. Effect of pCMV AIM-1 antisense Constructs on Human Ovarian Cancer Xenografts Growth in Nude Mice[J]. Cancer Research on Prevention and Treatment, 2015, 42(11): 1086-1090. DOI: 10.3971/j.issn.1000-8578.2015.11.006

Effect of pCMV AIM-1 antisense Constructs on Human Ovarian Cancer Xenografts Growth in Nude Mice

  • Objective To explore the effect of pCMV AIM-1 antisense constructs on human ovarian cancer xenografts growth in nude mice. Methods pCMV empty plasmids and pCMV AIM-1 antisense constructs were transfected into human ovarian cancer cells A2780, then the cells were trypsinized and expanded, and stable transfectants were generated after 400μg/ml geneticin(G418) screening. The clones carrying antisense plasmid were then selected for further experiments. RT-PCR and Western blot were used to examine the expression of AIM-1 mRNA and protein in three groups(each n=8); A2780 control group, A2780-pCMV empty plasmids and A2780-pCMV AIM-1 antisense group. Mouse tumor model was established by transferring human ovarian cancer cells A2780 or A2780-pCMV empty plasmids or A2780-pCMV AIM-1 antisense subcutaneously into the oxter of nude mice. The tumor growth, volumes and weights of subcutaneous tumor xenografts were detected. The expression of AIM-1 was detected by Streptavidinbiotin peroxidase(SP) immunohistochemical technique. Results (1)The mRNA and protein expression levels of AIM-1 were obviously silenced in A2780-pCMV AIM-1 antisense group, while no change in other two groups; (2)The tumor weight in A2780 control, A2780-pCMV empty plasmids and A2780-pCMV AIM-1 antisense groups were (1.93±0.21), (1.90±0.39) and (0.77±0.34)g, respectively; The tumor volume were (1 011.7±235.6), (981.2±162.3) and (468.7±119.6)mm3, respectively. There were significant differences in tumor weight and volume between A2780-pCMV AIM-1 antisense group and other two groups(P<0.01). The anti-tumor rate of pCMV AIM-1 antisense group could reach to 53.8%. The AIM-1 expression in A2780-pCMV AIM-1 antisense group was radically reduced, compared with A2780-pCMV empty plasmids and A2780 control groups. The positive rate and positive labeling index(PLI) of AIM-1 protein expression in A2780-pCMV AIM-1 antisense group were significantly lower than those in other two groups(P<0.01). Conclusion The pCMV AIM-1 antisense constructs suppresses the tumor xenografts growth in nude mice.
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