Effect of CRM1 Inhibitor KPT-185 on Growth of Human Prostate Cancer Cells

Objective To investigate the effect of chromosome maintenance region 1(CRM1) inhibitor KPT-185 on the growth, migration and apoptosis of prostate cancer cells and related reasons. Methods MTT assay, Clonogenic assay, Wound healing assay, flow cytometry analysis, Histone/DNA ELISA, Annexin V-FITC method and Western blot were used to detect KPT-185 effect on cell growth, migration, cell cycle, apoptosis, protein expression in prostate cancer cells and the potential mechanism, respectively. Results KPT-185 induction of cell apoptosis, growth inhibition and cell cycle arrest was involved in CRM1 inhibition and nuclear retention of tumor suppressor proteins p53 and p21 in prostate cancer cells. Conclusion KPT-185 as a new CRM1 inhibitor could be a potential drug in the treatment for prostate cancer.