Tanshinone ⅡA Inhibits Nerve Growth Factor Expression in Rats with Oxaliplatininduced Peripheral Neuropathy

Objective To observe the inhibitory effect of Tanshinone ⅡA on the expression of nerve growth factor in rats with oxaliplatin-induced peripheral neurotoxicity(OXIPN). Methods Thirty healthy Wistar rats were randomly divided into three groups, normal control group, model group and Tanshinone ⅡA group. Except the control group, other Wistar rats were injected intraperitoneally with Oxaliplatin (20mg/kg). And the rats in Tanshinone ⅡA group were injected intraperitoneally with Tanshinone ⅡA from 3d before modeling to 7d after that; moreover, electrophysiology was used to examine the mechanical threshold and cold allodynia; Western blot was employed to detect the expression of nerve growth factor(NGF) in dorsal horn and dorsal root ganglion. Results Oxaplatin could reduce the mechanical threshold and cold allodynia at 6h after modeling(P<0.05); the mechanical threshold value was decreased with the extension of observation, and reached the lowest level at 72h after modeling, which continued for 1 week. The expression of NGF in dorsal horn and dorsal root ganglion of model groups rats were significantly lower than those in control group at 7d after modeling; compared with model group, the mechanical threshold of rats were increased in TanshinoneⅡ A group, the tolerance of cold stimulation were prolonged, at meanwhile, the expression level of NSF was significantly up-regulated (P<0.05). Conclusion Tanshinon ⅡA could attenuated the oxaliplatin-induced neuropathic pain via increasing NGF level.