MicroRNA-184 Expression in Glioma Tissues and Its Prognosis Value

Objective To investigate the relationship between microRNA-184(miR-184) expression and clinicopathologic factors in glioma formalin-fixed paraffin-embedded(FFPE) tissues, and to evaluate the role of miR-184 expression in predicting the prognosis of glioma patients. Methods The expression levels of miR-184 were analyzed in 108 gliomas FFPE and 32 normal brain tissues by microarray and real-time PCR (RT-PCR). Survival analysis by Kaplan-Meier method was performed to assess prognostic significance. Results Down-regulation of miR-184 was detected in most primary glioma tissues, which was confirmed by RT-PCR analysis further. Additionally, down-regulation of miR-184 was significantly associated with WHO grade(P=0.007), Karnofsky performance score(KPS)(P=0.029), time to recurrence(P=0.037) and survival time(P=0.019). Using Kaplan-Meier analysis, a comparison of survival curves of low high expresser of miR-184 revealed a highly significant difference in overall survival(OS)(P=0.001) and disease-free survival(DFS)(P=0.006), which suggested that low expression of miR-184 was associated with a worse prognosis. Multivariate analyses showed that miR-184 expression was the independent risk factors for predicting OS hazard ratio(HR), 7.52; 95%CI: 2.63-21.42; P=0.002 and DFSHR, 11.56; 95%CI: 5.17-25.93; P<0.001 of glioma patients. Conclusion miR-184 expression is significantly correlated with the prognosis of glioma patients, suggesting that miR-184 may serve as the independent prognostic marker.