Anti-glioma Effect of Dendritic Cells Modified by RPL8 Gene

Objective To investigate the anti-glioma effect of dendritic cells(DC) modified by RPL8 gene. Methods DC were infected with the recombinant adenovirus Ad-RPL8. Green fluorescent protein was detected by fluorescence microscopy. Expression of RPL8 mRNA in DC was analyzed by RT-PCR. After the treatment with DC modified by RPL8 gene, the suppression of T cells on GL261 glioma cells was detected by MTT assay. The life span of mice and the pathology morphology of the tumor were analyzed after the mice were injected with DC. Results After infection with Ad-RPL8, green fluorescent and RPL8 mRNA expression were showed. T cells inhibited the growth of GL261 cells after stimulated by DC modified by RPL8 gene. The inhibition rate of T cells on GL261 cells were 78%, 49% and 43% in Ad-RPL8, Ad and PBS group, respectively. The inhibition rate was higer in Ad-RPL8 group than that in Ad and PBS group(P<0.05). The life span of Ad-RPL8 group was significantly prolonged and tumor volume was decreased, compared with Ad and PBS group(P<0.05). Conclusion The DC modified by RPL8 gene could activate T cells to suppress the growth of glioma, which could provide a new method for immunological therapy on glioma.