DNA Methylation Profile of Tumor Suppressor Genes in Diagnosis of Primary Hepatocellular Carcinoma

WANG Yongqiang; YE Ting; LIU Jinbo

doi:10.3971/j.issn.1000-8578.2014.12.017

Cancer Research on Prevention and Treatment > 2014 > 41(12): 1330-1334. > DOI: 10.3971/j.issn.1000-8578.2014.12.017

WANG Yongqiang, YE Ting, LIU Jinbo. DNA Methylation Profile of Tumor Suppressor Genes in Diagnosis of Primary Hepatocellular Carcinoma[J]. Cancer Research on Prevention and Treatment, 2014, 41(12): 1330-1334. DOI: 10.3971/j.issn.1000-8578.2014.12.017

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DNA Methylation Profile of Tumor Suppressor Genes in Diagnosis of Primary Hepatocellular Carcinoma

1. Department of Clinical Laboratory, The Second People's Hospital of Neijiang, Neijiang 6411002, China; 2. Department of Clinical Laboratory, The Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China

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Received Date: February 10, 2014
Revised Date: June 30, 2014

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Abstract

Abstract

Objective To investigate the value of promoter methylation status of cancer suppressor genes, such as beclin 1, RASSFA-1, p16, DAPK, etc., and the joint detection in the screening and early diagnosis of hepatocellular carcinoma(HCC). Methods Methylation-specific PCR (MSP) method was used to detect the promoter methylation status of 37 HCC samples and the corresponding tumor adjacent specimens. The area under the ROC curve (AUC) of beclin 1, RASSFA-1, p16, DAPK and stepwise LR results were compared by sensitivity, specificity, Youden index and positive/negative likelihood ratio. Results The positive rates of promoter methylation of beclin 1, RASSFA-1, p16 and DAPK genes in HCC were 5.4% (2/37), 94.6% (35/37), 73.0% (27/37) and 35.1% (13/37), Methylation of RASSFA-1, p16 and DAPK genes was more frequent in HCC than that in adjacent tissues(P<0.05). Combined detection of RASSFA-1 promoter methylation and AFP tumor marker could significantly increase the sensitivity (95.0%) and specificity (97.3%) and the area of ROC(0.903)in the diagnosis of HCC, and obtain higher sensitivity, Youden index and positive/negative likelihood ratio than single gene methylation. Conclusion Joint detection of RASSFA-1 promoter methylation and AFP tumor marker may become an effective index for screening and early diagnosis of HCC.
- Hepatocellular carcinoma,
- Methylation,
- Tumor suppressor,
- Tumor tissues

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