Effect of ABCG2 Expression on Chemoresistance of Pancreatic Carcinoma Cell Line SW1990

Objective To explore the relationship between chemoresistance to gemcitabine and ABCG2 expression in pancreatic carcinoma cell line SW1990. Methods The pancreatic carcinoma cell line SW1990 was cultured by DMEM and treated with 0.82 mg/ml gemcitabine for 24, 48 and 72 h. Then apoptosis rate was measured by flow cytometry, ABCG2 expression was examined by Western blot and ABCG2 mRNA expression was detected by RT-PCR. The relationship between ABCG2 expression and chemoresistance to gemeitabine was analyzed. Results After treated with 0.82 mg/ml gemeitabine for 24, 48 and 72 h, the apoptosis rates were(21.1±0.61)%, (13.4±2.17)%, (6.4±1.34)%, respectively (comparison between different time points, P<0.05); moreover, compared with control group, ABCG2 mRNA expression were increased by(2.21±0.11), (3.30±0.08) and (4.72±0.12) times and the protein expression were increased by(2.17±0.14), (3.61±0.09) and (4.98±0.13) times respectively (comparison between groups, P<0.05). Conclusion Gemcitabine could inhibit cell proliferation of pancreatic carcinoma cell line SW1990, but the chemoresistance would be enhanced gradually with time, which may be related with increased ABCG2 expression.