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KANG Yani, LAI Dengpan, ZHANG Xiaoli, CHEN Jian, ZHAO Xiaodong. CpG Islands Methylation Status in SHP1 Gene Promoter Region in Gastric Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2014, 41(08): 871-875. DOI: 10.3971/j.issn.1000-8578.2014.08.003
Citation: KANG Yani, LAI Dengpan, ZHANG Xiaoli, CHEN Jian, ZHAO Xiaodong. CpG Islands Methylation Status in SHP1 Gene Promoter Region in Gastric Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2014, 41(08): 871-875. DOI: 10.3971/j.issn.1000-8578.2014.08.003
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CpG Islands Methylation Status in SHP1 Gene Promoter Region in Gastric Cancer Cells

  • 1.School of Biomedical Engineering and Bio-ID Research Center, Shanghai Jiao Tong University, Shanghai 200240, China;2.Key Laboratory of Systems Biomedicine, Shanghai Jiao Tong University, Ministry of Education

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  • Objective To investigate the aberrant DNA methylation status of CpG islands in SHP1 gene promoter region in gastric cancer cells. Method CpG islands in the region of -1 000 bp-1 340 bp in transcription start sites of SHP1 gene were analyzed by Cpgplot software, and the primers of methylationspecific PCR (MSP) were designed by MethPrimer software. A new bisulfite-based MSP method was applied to detect the methylation status of SHP1 gene promoter region the limited gastric cancer cells. Bisulfite-modified DNA samples were amplified by MSP primers covering the CpG islands in SHP1 gene promoter region. The MSP products were purified cloned, which was subject to Sanger sequencing. Original and bisulfite modified partial sequences were compared. CpG loci methylation status was analyzed. Results Cpgplot displayed that MSP primers were located in SHP1 gene exon1 area which was close to the transcription initiation site. CpG islands length was > 200 bp, GC content was > 50%, and Obs/Exp was > 0.60. MSP detection showed that methylation-specific primer amplification was positive, whereas the unmethylation-specific primer amplification was negative, which revealed the region was highly methylated. Conclusion CpG islands in SHP1 gene promoter region are hypermethylated in gastric cancer cells.
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  • [1]
    Sandoval J, Heyn H, Moran S, et al. Validation of a DNA methylation microarray for 450,000 CpG sites in the human genome[J]. Epigenetics,2011,6(6):692-702.
    [2]
    Esteller M. CpG island hypermethylation and tumor suppressor genes: a booming present, a brighter future[J].Oncogene,2002,21 (35) :5427-40.
    [3]
    Oka T, Ouchida M, Koyama M, et al. Gene silencing of the tyrosine phosphatase SHP1 gene by aberrant methylation in leukemias/lymphomas[J].Cancer Res,2002,62(22):6390-4.
    [4]
    Kumagai C, Kalman B, Middleton FA, et al. Increased promoter methylation of the immune regulatory gene SHP-1 in leukocytes of multiple sclerosis subjects[J].J Neuroimmunol,2012,246(1-2): 51-7.
    [5]
    Capello D,Gloghini A,Baldanzi G,et al.Alterations of negative regulators of cytokine signalling in immunodeficiency-related non-Hodgkin lymphoma[J]. Hematol Oncol,2013,31(1):22-8.
    [6]
    Khan H,Vale C, Bhagat T, et al. Role of DNA methylation in the pathogenesis and treatment of myelodysplastic syndromes[J]. Semin Hematol,2013,50(1):16-37.
    [7]
    Li LC, Dahiya R. MethPrimer: designing primers for methylation PCRs[J]. Bioinformatics,2002,18(11): 1427-31.
    [8]
    Bock C, Reither S, Mikeska T, et al. BiQ Analyzer: visualization and quality control for DNA methylation data from bisulfite sequencing[J]. Bioinformatics, 2005,21(21):4067-8.
    [9]
    Barekati Z, Radpour R, Kohler C, et al. Specificity of methylation assays in cancer research: a guideline for designing primers and probes[J].Obstet Gynecol Int,2010,2010.
    [10]
    Braggio E, Maiolino A, Gouveia ME,et al. Methylation status of nine tumor suppressor genes in multiple myeloma[J]. Int J Hematol, 2010,91(1):87-96.
    [11]
    Wu C, Sun M, Liu L,et al.The function of the protein tyrosine phosphatase SHP-1 in cancer[J]. Gene,2003,306:1-12.
    [12]
    Minoo P, Zadeh MM, Rottapel R, et al. A novel SHP-1/Grb2- dependent mechanism of negative regulation of cytokine-receptor signaling: contribution of SHP-1 C-terminal tyrosines in cytokine signaling[J]. Blood,2004,103(4):1398-407.
    [13]
    Chim CS, Fung TK, Cheung WC, et al. SOCS1 and SHP1 hypermethylation in multiple myeloma: implications for epigenetic activation of the Jak/STAT pathway[J]. Blood, 20 04,103(12):4630-5.
    [14]
    Xu SB, Liu XH, Li BH, et al.DNA methylation regulates constitutive expression of Stat6 regulatory genes SOCS-1 and SHP-1 in colon cancer cells[J]. J Cancer Res Clin Oncol, 20 09,135(12):1791-8.
    [15]
    Ku JL, Jeon YK, Park JG. Methylation-specific PCR[J]. Methods Mol Biol,2011,791:23-32.
    [16]
    Herman JG, Graff JR, Myöhänen S, et al. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands[J]. Proc Natl Acad Sci U S A,1996,93(18):9821-6.
    [17]
    Min SY, Kim HS, Jung EJ, et al. Prognostic significance of glutathione peroxidase 1 (GPX1) down-regulation and correlation with aberrant promoter methylation in human gastric cancer[J]. Anticancer Res, 2012,32(8):3169-75.
    [18]
    Ye T, Chen Y, Fang J. DNA methylation biomarkers in serum for gastric cancer screening[J]. Mini Rev Med Chem,2010,10(11):1034-8.
    [19]
    Takamaru H, Yamamoto E, Suzuki H, et al. Aberrant methylation of RASGRF1 is associated with an epigenetic field defect and increased risk of gastric cancer[J].Cancer Prev Res(Phila), 20 12,5(10):1203-12.
    [20]
    To KF, Leung WK, Lee TL, et al. Promoter hypermethylation of tumor-related genes in gastric intestinal metaplasia of patients with and without gastric cancer[J]. Int J Cancer, 2002,102(6):623-8.
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