Construction and Digestion of Lymphoma-specific and High Efficient Gene Expression Vector survivin-VISA

Objective To construct lymphoma-specific and high efficient gene expression vector composed of the survivin promoter in the VP16-GAL4-WPRE integrated systemic amplifier system (S-VISA). Methods Three vectors, S-WPRE, S-TSTA and S-VISA, were constructed by gene recombination technology, comprised of different elements of woodchuck hepatitis post-transcriptional regulatory element(WPRE), two-step transcriptional amplification(TSTA) and VISA under control of survivin promoter, respectively; PCR method and enzyme digestion were used to prove its correctness. Lymphoma cell lines Ramos, U937, Raji and hepatic cell line Chang Liver were transfected with these vectors by liposome to detect the luciferase activities. Results Expression vectors S-WPRE, S-TSTA and S-VISA were proved to be constructed correctly by PCR and enzyme digestion. Luciferase activity analysis showed that S-VISA achieved lymphoma-specific and high efficient expression of the target gene. Conclusion Lymphomaspecific and high efficient gene expression vector S-VISA is constructed successfully, which laid a good foundation for further study of lymphoma gene therapy.