Effects of miR-205 Overexpression on Cisplatin-induced Apoptosis of Adrenocortical Carcinoma Cell Line SW-13 and Its Mechanism

Objective To investigate the effect of miR-205 overexpression on apoptosis of adrenocortical carcinoma cell line SW-13 induced by cisplatin (CDDP) and to explore the related mechanism. Methods Two kinds of adrenocortical carcinoma cell lines SW-13, one transfected with pcDNA3.1( +)-miR-205(treatment group) and the other transfected with pcDNA3.1( + ) (control group), were involved. With different concentrations of cisplantin, sensitivities of two groups to different doses of CDDP were tested by CCK-8 assay, apoptosis was examined by the staining of H33342 and AnnexinV-FITC/PI, AnnexinVPE/7AAD flow cytometry was applied to assess apoptosis rates, expression levels of apoptosis-related proteins were detected by Western blot, and mRNA expression levels of E2F1 and VEGF-A were investigated by qPCR. Results The apoptosis and growth inhibition rates in treatment group were significantly higher than those in control group（P＜0.05, P＜0.01）. After treated with 0, 20, 30μg/ml of CDDP, Bcl-2 expression and mRNA expression of E2F1 and VEGF-A in treatment group were decreased, while Bax, Caspase-9 expression in treatment group were markedly increased. Conclusion MiR-205 overexpression in SW-13 would down-regulate E2F1 and VEGF-A expression levels, enhance the sensitivity of adrenocortical carcinoma cells to cisplantin, and promote cell apoptosis.