Correlation of Dynamic Changes of Candidate Plasma Proteins Expression with Development of Cervical Carcinogenesis in Uyghur Women

Objective To explore the relationships of the dynamic changes of candidate plasma proteins expression with the development of cervical carcinogenesis in Uyghur women based on the previous plasma proteomic research. Methods We collected 82 cases of plasma samples from Uyghur women, 20 cases with cervicitis (CV), 20 cases with cervical intraepithelial neoplasia (CIN) Ⅱ-Ⅲ, 20 cases with early stage (Ⅰ-Ⅱ) and 20 cases with advanced stage (ⅡB-Ⅳ) cervical squamous cell carcinoma, quantitatively determined the content of plasma proteins ATⅢ, VIL1 and CLU by enzyme linked immunosorbent assay (ELISA) and statistical analysis, and evaluated the specifi city and sensitivity of candidate plasma protein markers. Results With the development of cervicitis to CIN Ⅱ-Ⅲ, early stage or advanced stage cervical cancer, the changes of these plasma proteins altered gradually, with remarkably decrease in the content of ATⅢ, and increase of VIL1 and CLU, with statistical differences (P<0.05). Compared with cervicitis group, there were signifi cant differences of the content of plasma proteins ATⅢ, VIL1 and CLU in CINⅡ-Ⅲ, early stage or advanced stage cervical cancer group (P<0.05). Correlation analysis of these three protein indicators showed no correlation (P>0.05). By the detection of ATⅢ, VIL1 and CLU in the cervical lesions, generally the sensitivity, specifi city and accuracy of the ATⅢ protein were the highest (73.0%, 72.0%, 73.0 %), VIL1 were the next (65.0%, 57.0%, 54.0%), and CLU were the lowest (60.0%, 59.0%, 59.0%). Conclusion The content of plasma proteins ATⅢ, VIL1 and CLU is an important symbol of the process of cervical lesions, and has high predictive value. It is expected to be the early diagnosis of cervical cancer.