Graphene Oxide Absorbed 5-Fu, Antibody and Cytokine Are Bioactive in vitro

Objective To investigate whether Graphene Oxide (GO) absorbed chemotherapeutic drug and immune stimulators are bioactive in vitro. Methods Graphene Oxide was used to absorb 5-Fu, IFNγ and anti-IL10R antibodies respectively. The biological characteristics of absorbed 5-Fu, IFNγ and anti-IL10R antibodies were examined by microscope, ELISA and Western blot, etc. in vitro. Results GO itself does not kill tumor cells, and does not promote CD11c+ dendritic cells upgrade CD86 expression on their cell membrane. GO absorbed 5-Fu is able to kill tumor cells in vitro, and the killing of tumor cells is dose dependent. GO absorbed 5-FU kills tumor cells more effi ciently than free 5-Fu. GO absorbed IFNγ and anti-IL10R antibodies are able to enhance IL12 secretion by CD11c+ dendritic cells stimulated by LPS. Conclusion Graphene oxide absorbed chemotherapeutic drug and immune stimulators are bioactive in vitro and may provide a new way for cancer therapy.