Effects of Lentiviral-Mediated Foxp1 siRNA to Proliferation, Apoptosis and Migration of Hepatoma Carcinoma Cell

Objective To explore the effects of Foxp1 on proliferation, apoptosis and migration of hepatoma carcinoma cells. Methods Small nucleic acid fragments which could interfere Foxp1 (siRNA Foxp1) were synthesized in vitro, and it was inserted into the transfer plasmid of the lentiviral three-plasmid system by recombinant techniques, using the packaging cells (293T) to assemble the three-plasmid system as a complete retrovirus lentivirus vector (lenti-pLL3.7-Foxp1-siRNA), which was used to infect hepatoma carcinoma cell lines with high expression of Foxp1; at the same time, the empty viral vector without the the Foxp1-siRNA sequence directly packaged by three-plasmid system was constructed as control group. The infected effect of lentiviral vector-mediated siRNA was detected by fl uorescence microcopy. Expression of Foxp1 in hepatoma carcinoma cells were detected by western blot and real-time QPCR (RT-QPCR) at protein and mRNA levels. CCK-8 experiments, fl ow cytometry, the wound healing assay and transwell assay were used to investigate the changes in cell proliferation, apoptosis and migration. Results Compared with control group, expression of Foxp1 protein and mRNA significantly decreased in hepatoma carcinoma cells infected with lentipLL3.7-Foxp1-siRNA; meanwhile, the vitality of cells was notably inhibited, and the apoptosis strikingly increased, the migration in the two-dimensional space and three-dimensional space signifi cantly decreased (respectively, P<0.01). Conclusion As a kind of multi functional transcription factor, Foxp1 could promote the proliferation and migration of hepatoma carcinoma cells, and inhibit its apoptosis.