Effect of Exogenous AES on Malignant Behaviors of Colorectal Cancer Cells

Objective To study the infl uence of amino-terminal enhancer of split(AES) on malignant behaviors of colorectal cancer(CRC). Methods LoVo has a lower expression of AES than other CRC cell lines conserved by our laboratory.The pEGFP-aes construct was transfected into LoVo cells by lipofectamine TM2000 (pEGFPAES). Cell Counting Kit-8 was applied to analyze cell proliferation. The cell invasion and migration abilities were examined by Transwell assay and wound healing assay respectively. Results Contrast to pEGFP-N1(transfect pEGFP-N1 into LoVo cells), AES can significantly repress cancer cells invasion and migration(P<0.05),but had no influence in cell growth and proliferation. Conclusion AES effects CRC's malignancy by repressing its invasion and migration.