Effects of Gold Nano Particles on HIF-1α and VEGF mRNA Expression and Normalizing Vascellum in Hepatic Tumor of Nude Mice

Objective To observe the effects of GNP (goldnano particles,GNP) on nude mice hepatic tumor vascular morphology and the expression of hypoxia inducible factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF) mRNA. Methods Hepatocellular cancer cell line H22 were injected subcutaneously into the right armpits of 14 Balb/c nude mice. When the diameter of transplanted tumor reached about 7-8 mm,they were randomly divided into 2 groups, and drugs with GNP or normal saline were injected respectively. For 7 days later, the tumor vascular diameter and blood volume were measured by Doppler ultrasound. When the mice were sacrificed, the tumor's size was calculated and the expression of HIF-1α and VEGF mRNA were detected by in situ hybridization. Results (1)The tumor's size in experiment group (0.935±0.129 cm³) was decreased in comparison with that in control group (1.573±0.247 cm3), there was significantly difference (P<0.05).(2) The tumor's vascular diameter and blood volume in experiment group were (0.6397±0.1548)mm and (1.171±0.241)cm³ respectively, they were all signifi cantly reduced in comparison with those in control group (1.1000±0.3247 mm, 2.357±0.408 cm3)(P<0.05). (3)The expression of HIF-1α mRNA (15.3±7.4%) and VEGF mRNA (23.7±9.5), were dramatically attenuated in comparison with those in control group (67.2±13.1)%,(70.3±14.6)% (P<0.05). Conclusion GNP can make nude mice tumor's vascular morphology better and decrease their blood volume, inhibit the expression of HIF-1α mRNA,VEGF mRNA, this situation is benefi t to restrain the tumor growth.