Effects of Arenaria Kansuensis Aqueous Extract on Proliferation and Cell Cycle of Human Gastric Cancer Cell Line MGC-803

WANG Gang; ZHANG Hua; YANG Xinrui; WANG Haiyan; HE Juxiang; JI Libin

doi:10.3971/j.issn.1000-8578.2013.09.001

Cancer Research on Prevention and Treatment > 2013 > 40(09): 821-825. > DOI: 10.3971/j.issn.1000-8578.2013.09.001

WANG Gang, ZHANG Hua, YANG Xinrui, WANG Haiyan, HE Juxiang, JI Libin. Effects of Arenaria Kansuensis Aqueous Extract on Proliferation and Cell Cycle of Human Gastric Cancer Cell Line MGC-803[J]. Cancer Research on Prevention and Treatment, 2013, 40(09): 821-825. DOI: 10.3971/j.issn.1000-8578.2013.09.001

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Effects of Arenaria Kansuensis Aqueous Extract on Proliferation and Cell Cycle of Human Gastric Cancer Cell Line MGC-803

1. Department of Pathology, Medical College of Qinghai University, Xining 810001, China; 2.Department of Function, Huangshi Second Hospital

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Received Date: January 02, 2013
Revised Date: June 17, 2013

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Abstract

Objective To investigate the effects of arenaria kansuensis aqueous extract (AKAE) on proliferation and cell cycle of human gastric cancer cell line MGC-803. Methods MGC-803 cells were treated with AKAE at different concentration. The effect on proliferation of MGC-803 cells was detected by MTT assay. The flow cytometry was used to test the cell cycle of MGC-803 cells. The protein and mRNA expressions of cyclin D1, p16 and p21 in MGC-803 cells were detected by western blot and real-time PCR respectively. Results The proliferation of MGC-803 cells was dose-dependently inhibited by AKAE treatment in vitro(P<0.05)，and the value of IC₅₀ was (0.134±0.005)mg/ml. After treated with AKAE at the dose of 0.8 mg/ml for 12 h, the ratio of G₁ phase in MGC-803 cell cycle was increased, and the ratio of S phase in MGC-803 cell cycle was decreased(P<0.05). The protein and mRNA expressions of cyclin D1 in MGC-803 cells were obviously blocked by AKAE at the dose of (0.2-0.8) mg/ml（P<0.05）.AKAE treatment could enhance the mRNA expressions of p16 and p21 in MGC-803 cells.Compared with control group, the relative expression values of p16 and p21 mRNA in 0.2 mg/ml group were 2.30 and 17.5 times higher, respectively. Conclusion AKAE could inhibit the proliferation of MGC-803 cells, and the mechanism is closely associated with G₁-phase cell cycle arrest and expression of G₁-phase related factors.
- Arenaria kansuensis,
- MGC-803 cells,
- Proliferation,
- Cell cycle

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