Effects of Small Interfering RNA Specific to Stable Transfection Gene of PGRMC1 on Proliferation of Ovarian Carcinoma in vitro

Objective To investigate the effects of the proliferation of the human ovarian cell line (COC1) by stable transfection short hairpin RNA into the target PGRMC1 gene. Methods The text subjects were divided into three groups. PGRMC1 recombinant expression plasmid was constructed and then was transfected into COC1 cells by lipofectamine 2000. The mock-vehicle group only transfected the blank load pcDNA3.1 A plasmid. And the blank group was composed of non-transfected plasmids. The three groups gained stable-transfected cell lines after resistance selection. The expression of PGRMC1 mRNA was tested by reverse transcription RT-PCR.The proliferation of COC1 cells after PGRMC1 gene transfection was measured by methylthiazolyl tetrazolium(MTT). And cells growth curves were plotted. The tumor growth of the nude mice inoculated of tumor cells was compared with before and after transfection. Results The results of RT-PCR showed that the significant expressions of PGRMC1 mRNA after infected by PGRMC1 plasmid were higher than those in the control. The COC1 cell growth in stable transfection status was more significantly decreased than that in non-transfection status (P<0.05). After inoculating transfected cells into nude mice,it took (5.8±0.7 )days to grow the planed tumors in group infected by PGRMC1 plasmid. The days were significantly shorter than those in the control(11.9±0.5)days and in the blank group (12.6±0.9) days (P<0.05). There were no significant differences between the mock-vehicle group and the blank group (P>0.05). And five weeks after inoculation, mice tumors' weights and sizes of the recombinant vectors group were (0.7±0.4) g and (197±26) mm³ respectively, which were significantly lower than those of the mock-vehicle group (2.3±0.4) g and (785±38)mm³ and those of the blank control group(2.5±0.8) g and (896±22)mm³. Conclusion PGRMC1 siRNA could remarkably reduce the expression of PGRMC1 gene in ovarian cell and also inhabit the ovarian cell growth.