Detection of LOH on 3 p14.2-14.1 in Esophageal Squamous Cell Carcinoma

Objective To search for small overlap missing area on the third chromosome short arm in esophageal squamous cell carcinoma (ESCC) so as to provide the experimental basis for screening tumor ESCC related suppressor genes. Methods Microsatellite analysis method was used to detect the heterozygosity lost (LOH) of ESCC and normal tissue adjacent to the cancer. Results Five microsatellite markers distributing on 3 p21.1-p13 area showed high frequency change,chain analysis indicated that there was a small overlap missing area in 3 p14.2-p14.1,identified as the markers D3S2452,D3S3040 and D3S1766,encompassing approximately 283 kb long. Conclusion There might be some new tumor suppressor genes in 3p14.2-p14.1 area.It could be valuable to further investigation for the related genes of ESCC in the area.