Effect of Rapamycin on Proliferation of Human Cervical Carcinoma HeLa Cell and Expression of HIF-1α and VEGF

Objective To explore the effects of different concentrations of rapamycin on growth inhibition and influence on HIF-1 alpha, VEGF expression in HeLa cervical cancer cells. MethodsUsing four methylazo thiazole blue (MTT) colorimetric method to detect the inhibition ratio of in vitro human cerical cancer HeLa cells processed with different concentraions of rapamycin, the reverse transcription polymerase chain reaction (RT-PCR) and Western blot were used to detect mRNA and protein levels of HIF-1 alpha and VEGF. Results Different concentrations of rapamycin inhibited HeLa cervical cancer cells growth signnificantly, with a dose-dependent mode; with increasing concentration of rapamycin, both mRNA and protein levels of HIF-1 alpha, VEGF were reduced. Conclusion Rapamycin inhibited HeLa cervical cancer cells growth with a dose-dependent mode, and rapamycin obviously down-regulated HIF-1 alpha, VEGF at both mRNA and protein levels.