Establishment and Characterization of Human Immortalized Melanocytoma Cell Line

ObjectiveTo establish a human immortalized melanocytoma cell line and explore its biological properties. Methods Meningeal melanoma tissue was isolated to primary and mass culture,to establish a immortalized cell line and to detect its biological characteristics.Cell morphology and ultra-structure were studied through HE staining,inverted microscopy and transmission electron microscopy.Growth curve and doubling time of cells were calculated by cell counting.Karyotype (chromosome banding assay) and tumorigenesis in animals were also detected.Vimentin,S100,HMB45 and CK protein expression were measured by SP immunohistochemical method. Results Clinicopathological diagnosis was primary intracranial melanoma.The melanoma cell line established from melanoma tissue,showed an epithelioid cell growth with intracellular melanin granules,visible cell clone colony and melanosomes;detected by transmission electron microscope.The cell line had a doubling time of 36h,and the chromosome number was 32 to 110.Cell line transplanted into nude mice could not survival and cell line had positive Vimentin expression,and negative HMB45,S100 and CK expression. Conclusion We established a human immortalized melanocytoma cell line with benign biological characteristics.