Gene-environment Analysis on Association of Chromosome 8q24 and Chromosome 17q24 with Prostate Cancer in Chinese Population

Objective To explore whether the risk alleles in Chromosome 8q24(rs620861C,rs1447295A)and Chromosome 17q24(rs1859962G)are associated with prostate cancer in Chinese population;to investigate the association between genotypes and phenotypes(age,T staging of neoplasm,PSA level,Gleason scores and suffering from BPH or not)of PCa patients，to analyse the the interactions among the genes. Methods A case-control study was performed to collect information of PCa patients'clinical features.By using polymerase chain reaction-high resolution melting curve(PCR-HRM)combined with DNA sequencing,each locus was genotyped and examined,and the distribution of each locus' genotype was compared in an age-matched case-control method(124 cases,138 controls),and the gene-gene interaction analysis was done.The contribution of patients′ genotypes in different clinical features was done. Results None of the three variants was observed to show significant difference in the distribution of genotype and gene frequency between cases and controls,neither did they show significant association with PCa phenotypes in cases.There was no significant gene-gene interactions being detected,either. Conclusion Chromosome 8q24(rs620861C,rs1447295A)and Chromosome 17q24(rs1859962G)may have no association with prostate cancer in Chinese population.