Effects of Peptide 30 Derived from Endostatin on Proliferation,Invasion and Metastasis of Human Gastric Adenocarcinoma SGC-7901 Cells

Objective To study the effects of a 30-amino-acid N-terminal peptide of endostatin modified by RGD on the proliferation and metastasis of human gastric adenocarcinoma cell(SGC-7901). Methods The nucleotide sequence encoding amino acids 1-30 of endostatin(peptide 30,with amino acids 25-31 mutated from RGIRGAD to RGDRGD)was artificially synthesized and cloned into the plasmid pTYB2 and expressed in E.coli(DE3).WST-1 kit was used to evaluate the effects of peptide 30 on the growth of SGC-7901.Wound healing assay and Transwell assay were adopted to evaluate the effects of the peptide 30 on the migration of cancer cells.The expression and secretion of tumor metastasis-related protein MMP-2 (metalloproteinase-2)and MMP-9(metalloproteinase-9) were determined by gelatin zymography. Results Peptide 30 effectively suppressed the proliferation of SGC-7901 cells.Peptide 30 was found to effectively suppress the invasion and migration of SGC-7901 cells.Moreover,peptide 30 inactivated MMP-2 and MMP-9 activity. Conclusion The mechanism of peptide 30 suppressed cancer metastasis may involve the down-regulation of MMP-2 and MMP-9 activity.The peptide 30 can potentially be used to treat gastric carcinoma in clinic.