Expression and Significance of TRX-2 in Bone Marrow of Acute Leukemia

Luo Qiong; Chen Jing; Huang Zhixin; Du Xin

doi:10.3971/j.issn.1000-8578.2012.11.020

Cancer Research on Prevention and Treatment > 2012 > 39(11): 1361-1364. > DOI: 10.3971/j.issn.1000-8578.2012.11.020

Luo Qiong, Chen Jing, Huang Zhixin, Du Xin. Expression and Significance of TRX-2 in Bone Marrow of Acute Leukemia[J]. Cancer Research on Prevention and Treatment, 2012, 39(11): 1361-1364. DOI: 10.3971/j.issn.1000-8578.2012.11.020

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Expression and Significance of TRX-2 in Bone Marrow of Acute Leukemia

1.Department of Hematology,Guangdong Provincial People′ s HospitalGuangdong Academy of Medical Science,Guangzhou 510080,China,2.Research Center of Medical Sciences

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Received Date: February 21, 2012
Revised Date: June 25, 2012

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Abstract

Abstract

Objective To investigate the correlation between TRX-2 expressions level and clinicopathological charateristics of acute leukemia. Methods The expressions level of TRX-2 in bone marrow from 120 patients with acute leukemia were detected by RT-PCR(Real-time PCR) and indirect immunofluorescence,respectively,and followed by subsequent clinical characteristics correlative studies. Results By RT-PCR detecting,the expressions level of TRX-2 in 120 cases of acute leukemia were significantly higher than that of 120 other cases of free-blood disease［(5.08±0.52)×10²vs.(0.91±0.15)×10², P<0.05］.With respect to the percentage of blast cell in bone marrow,an increase trend of TRX-2 mRNA expressions in blast cell of bone marrow over 90% was found in comparison to patients with blast cell of bone marrow bellow 90%,and statistical difference was found between them ［(6.79±0.51)×10² vs.(3.26±0.58)×10²,P<0.05］.TRX-2 mRNA expressions level in bone marrow were not related to age,gender and classifications of acute leukemia.By indirect immunofluorescence detecting,TRX-2 protein in test group occure more frequently than that in control group.There was significant correlation between RT-PCR and indirect immunofluorescence (P<0.05),although with poor consistency(κ=0.26). Conclusion TRX-2 is high expression in acute leukemia and involved in the percentage of blast cell in bone marrow.The presence of TRX-2 appears not related to clinical characteristics.There is significant correlation between RT-PCR and indirect immunofluorescence,although they are poor consistency.
- Thioredoxin-2,
- Acute leukemia,
- Real-time PCR,
- Indirect immunofluorescence test

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References

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