Oct4 Binds to miRNA302 Cluster Promoter in F9 Mouse Embryonic Carcinoma Cell

Objective To investigate whether Oct4 binds to the miR302 cluster promoter in the F9 mouse embryonic carcinoma cell. Methods The expression of Oct4 and miR302 in F9 cells after Oct4 siRNA interference were analyzed by Real-time PCR.We examined the binding of Oct4 to the miR302 cluster promoter in F9 cells by ChIP using antibodies against Oct4.The purified DNA was amplified by PCR using miR302 promoter-specific primers. Results After siRNA-mediated knockdown of Oct4,Oct4 levels was decreased 56% and miR302 expression was decreased 38%,we found that Oct4 bound to the putative promoter region of the miR302 cluster. Conclusion These data indicate that Oct4 and miR302 are coordinately expressed in F9 cells,Oct4 binds to the miR302 cluster promoter in the F9 mouse embryonic carcinoma cell.The miR302 may be regulated by Oct4.