Effect and Mechanism of Plumbagin in Human Osteosarcoma Cell Line U2OS

Objective To explore the effect of plumbagin on the growth of osteosarcoma cell line U2OS and its possible underlying mechanisms. Methods Cell apoptosis of plumbagin against osteosarcoma was studied by CCK-8 assay；morphological changes was studied by HOECHST 33342 staining；the expression of p53 and MDM2 gene was studied by Western blot assay. Results CCK-8 assay showed that plumbagin had an obvious inhibition on osteosarcoma cell line in a dose-dependent manner；HOECHST 33342 staining showed that plumbagin inhibited the growth of osteosarcoma cell line U2OS through the promotion of apoptosis；Western blot assay showed that plumbagin played the role by changing the expression proportion of p53 and MDM2. Conclusion Plumbagin inhibited osteosarcoma cell line U2OS cells growth through cell apoptotic pathways.