Inhibitory Effects on HepG2 Cells from Genetic Engineering Cells Secreted Tumor Necrosis Factor

Objective To explore the effects of genetic engineering cells which secreted tumor necrosis factor(TNFα) co-cultured with human hepatic cancer cells HepG2 on the proliferation of cancer cells. Methods The expression and secreting of tumor necrosis factor was detected by RT-PCR,Western blot,ELISA and flow cytometry from the 293 cells which could stably secrete TNF-α.The inhibitory effects on the hepatic cancer cells HepG2 from the 293 cells (hTNFα/293) were detected by MTT assay under the wavenumber of 490 nm at different time. Results The results from RT-PCR,Western blot and ELISA indicated that hTNF-α /293 cells and TNF-α-positive group had significant inhibition on the proliferation of hepatic cancer cells in a dose-dependent manner. Conclusion The TNF-α/293 cells can effectively secrete TNF-α .And the TNF-α secreted from the genetic engineering cells has an inhibition on hepatic cancer cell proliferation in a dose-dependent manner.