Study of miRNA Signatures as Biomarkers for Early Stage Nonsmoking Female Lung Cancer of Xuanwei County of Yunnan Province

Objective To explore miRNAs that relative to tumorigenesis as biomarkers for early detecting nonsmoking female lung cancer of Xuanwei County. Methods (1) Detecting miRNAs expression in 37 cancer tissues in nonsmoking female lung cancer of Xuanwei County and 32 cancer tissues in individual cases by microarray hybridization.To validate the microarray analysis result by RT-qPCR.(2) Evaluating plasma expressions of the miRNAs in 16 stage Ⅰnonsmoking female lung cancer of Xuanwei County patients and 14 counterpart benign cases.Evaluating diagnostic sensitivity and specificity of the miRNA in plasma for nonsmoking female lung cancer of Xuanwei County by Receiver-Operator Characteristic curve（ROC）. Results There were 31 miRNAs differentially expressed in all tumor tissues compared to their corresponding normal lung cancer.The over expressions of the genes in tumors were confirmed by RT-qPCR.The miR-21,miR-10a,miR-494,miR-22,miR-141 and miR-200b expression levels between two cancer tissues were different（P<0.05）.The six miRNAs were stably present and reliably detectable in plasma.miR-494,miR-22 and miR -200b expression levels were significant different between two plasmas（P<0.01）.The use of the three genes produced 85.26% sensitivity and 94.45% specificity in distinguishing nonsmoking female lung cancer of Xuanwei County patients from the female crowd. Conclusion From express stability,sensitivity and specificity that the identified three miRNAs provide potential biomarkers for early detection of nonsmoking female lung cancer of Xuanwei County.