Association between Polymorphism of Heat-shock Protein A5 and HBV Invasion

Objective To explore the association between heat-shock protein A5(HSPA5) and hepatitis B virus(HBV) invasion. Methods DNA was genotyped for the single-nucleotide polymorphism(SNP) by polymerase chain reaction and sequencing in 210 unrelated HBV carriers and a total of 194 gender- and age-matched healthy controls.Serological markers for HBV infection were determined by enzyme-linked immunosorbent assay(ELISA) kits or clinical chemistry testing. Results The distributions of allele and genotype in cases were not significantly different from that in controls.In addition，neither ALT/HBeAg nor HBV-DNA was associated with the allele/genotype prevalence in HBV infected individuals. Conclusion -86 bp T/A polymorphism in HSPA5 gene is not related to the clinical risk and acute exacerbation of HBV invasion.