Effect of survivin Gene Silencing on Proliferation of Human Cervical Cancer Cell XB1702 and Their Sensitivity to Gefitinib

Objective To investigate the effect of small interfering RNA-mediated survivin knock-down on proliferation of human Cervical cancer Cell XB1702 and their sensitivity to Gefitinib. Methods The siRNA against survivin was constructed and transfected into XB1702 cells with LipofectamineTM 2000.The expression of survivin was detected by RT-PCR and Western blot.Flow cytometry was used to detect the cell cycle.Sensitivity to Geftinib after transfection were examined by MTT and clonogenic assay. Results In XB1702 cells,the protein and mRNA levels of survivin were decreased significantly after transfection,and reduction of proliferation was related to an increase in the fraction of G₀/G₁ phase.The sensitivity of XB1702 cells to Gefitinib increased significantly after transfection. Conclusion The survivin special siRNA silenced surviving,decreased XB1702 cells proliferation and enhanced their sensitivity to Gefitinib.