Effect of Xiaoaiping on Growth and Angiogenesis of H22 Hepatic Carcinoma in Mice

Objective To study the effects of Xiaoaiping(XAP) with different concentrations on the tumor angiogenesis in mice and to discuss its possible mechanism in the inhibition of hepatic cellular cancer growth. Methods Seventy-five female KM mice whose weight is about(20±2)g were injected H22 liver tumor cells.They were randomly divided into five groups as following:D1(5-fluorouracil(5-Fu) was peritoneal injected,as a positive control),D2(NS was peritoneal injected,as a negative control),groups X1,X2 and X3 were peritoneal injected with XAP of 10 g/(kg·d),20 g/(kg·d) and 40 g/(kg ·d) respectively.The tumor′s volume was calculated to draw the tumor growth curve.The tissue of xenografted tumors was taken after 3 week′s treatment.The xenografted tumors were weighted its inhibitory rate was calculated.The pathological changes were observed by light microscope.The microvessel density(MVD) marked by CD34 in the xenografted tumor tissue was detected by immunohistochemisty.The concentration of serum VEGF was measured by ELISA. Results The inhibition rate in X3 and D1 group was similar(P>0.05).The doses of serum VEGF in X1,X2 and X3 group were all lower than that in D2 group(P<0.05),especially in X3group.To detect MVD,the conditions were the same as the dose of serum VEGF. Conclusion Different doses of XAP can inhibit tumor growth of H22 liver cancer in mice and the medium dose of XAP has the best inhibition.XAP may reduce VEGF and decrease MVD in tumor to make tumor ischemia and necrosis in order to inhibit tumor growth.