Comparison between Two Methods of Establishing Induced Buckle Pouch Carcinogenesis Model in Syrian Golden Hamster

Objective To establish hamster buckle pouch carcinogenesis model with injection of DMBA acetone solution at different concentrations，and compares it with the traditional paint method. Methods Eighty one golden hamsters were assigned randomly into three groups: 60 hamsters in the injection groups were injected with DMBA acetone solution according at the concentrations of 0.5%，0.4%，0.3%，0.2%，0.1% respectively，twice a week， for 9 weeks.15 hamsters as the paint group were painted with 0.5% DMBA acetone solution，and 6 hamsters without any treatment belonged to the control group.General observation，weight，histopathology were executed every week.Three animals in every group were sacrificed by anesthesia at 3，6，9，12，18 weeks respectively and subjected to histological examination. Results The injection group with the concentration of 0.4%，0.3%，0.2%，0.1% appeared about 0.8 cm × 0.6 cm × 0.5 cm size of the tumor in 13，9，12，16 weeks respectively.The rate of carcinoma formation were 25%、87%、42% and 58% at 12 weeks.The mortality rate were 48%，11%，13% and 10% respectively.In the paint group tumor occurred in 18 weeks.The rate of carcinoma formation and mortality were 48% and 37% at 12 weeks. Conclusion The DMBA acetone solution at the concentration of 0.3% is more suitable for the formation of oral squamous cell carcinoma in golden hamster in the short term.This method is benefitial to shorten the feeding cycle，reducing the costs of the model.And it may provide an ideal animal model in vivo for further study.