Apoptosis of Cervical Cancer Cell Line HeLa Induced by MAC-1 Protein from Musca Domestica Larva Hemolymph

Objective To investigate the apoptosis-inducing effect and mechanism of the MAC-1 protein from Musca domestica larvae three age hemolymph extract on Cervical cancer cell line HeLa. Methods The morphology of apoptosis cells was observed with hematoxylin- eosin stainings and flow cytometry.The expression of Fas，caspase 8 and bcl-2 were examined by immunocytochemical staining Results. Results Treated with the MAC-1 protein from Musca domestica larvae three age hemolymph extract，the HeLa cells showed the morphological apoptosis;FITC/PI stained FCM showed the apoptosis rate of HeLa had been decreased along with the prolonging of time and the increase of the MAC-1 protein concentrations.With the MAC-1 protein，immunocytochermical staining showed the enhanced expression of Fas and caspase 8，and attenuated expression of bcl-2. Conclusion The MAC-1 protein from Musca domestica larvae three age hemolymph can induce HeLa cells，tapoptosis in- time and concentration -dependent manners，which may be related to the expression of Fas，caspase8 and bcl-2.