Interleukin 24 Inhibits Growth and Induces Apoptosis of Human Large Cell Lung Cancer Cell Line NCI- H460 in vitro

Objective To study the enhanced anticancer effect and its mechanism by Ad-polyA-promoer-IL-24（Ad-IL-24） for NCI-H460 cells. Methods Transfected into NCI-H460 cells with the different recombinant adenovirus，the expression of IL-24 was identified by Western blot.The influence of the cell growth was tested by MTT essay.The cell apoptotic effect was evaluated by flow cytometry(FCM)，which were dyed by Annexin-V-PE /7-AAD.The expressions of cytokines including bax，caspase-3，bcl-2 and survivin were explored by RT-PCR. Results Western blot proved that IL-24 was effectively transcribed in NCI-H460 cells.Ad-IL-24 can induce apoptosis by up-regulating bax，caspase-3 expression while down-regulating bcl-2 and survivin expression．Cell growth was obviously inhibited by the recombinant adenovirus. Conclusion It was proved that there was enhanced anticancer effect by Ad-IL-24.The mechanism may be related to the up-regulations of bax，caspase-3 and the down-regulations of bcl-2，survivin.