Effect of TIP30 on Biological Characters of HCT116 Cells

Objective To study the association of TIP30 and biological characters of HCT116 colorectal cancer cells. Methods The eukaryotic expression vector of TIP30 gene was established and transfected into HCT116 cells by liposome-mediated transfection.The expressions of TIP30 gene mRNA and protein were detected by RT-PCR and Western blot.The invasive ability was analyzed by Matrigel invasion assay.The colony formation assay was used to detect cell growth inhibition. Results The stable transfectant of HCT116/pCMV4-flag-TIP30 cell was established successfully,the proliferation of HCT116/pCMV4-flag-TIP30 cell was inhibited,the invasive ability and colony formation ability were also decreased compared to control cell. Conclusion The overexpression of TIP30 gene in HCT116 cells not only results in inhibiting cell growth and cell apoptosis,but also reducing cell migration and invasion,which provides evidence for TIP30 gene therapy.