Experiment of Vinorelbine or Combined with Hyperthermia on Inhibiting Angiogenesis

ObjectiveTo investigate the antiangiogenesis ability of Vinorelbine or combined with hyperthermia in vitro and in vivo. Methods Human pulmonary adenocarcinoma A549 cell was used as control; proliferations of human umbilical vein endothelial cell(HUVEC) was measured by MTT assay.Transwell cabin test and out-body canaliculization test were used to observe the impact of Vinorelbine on cell migration and capillary-like tube formation ability, and the apoptosis rate of HUVEC was calculated by flow cytometry.In vivo, the chicken chorioallantoic membrane (CAM) model was used to check whether the neovascularization of CAM could be suppressed. Results Low dose Vinorelbine ［(0.1～1)ng/ml］ had a much more effect of inhibiting the proliferation of HUVEC than that of A549, and the difference was significant (P=0.000).The inhibition rate of HUVEC within a range of （0.1～1.0）ng/ml Vinorelbine was positive correlated with the concentration (r=0.993, P=0.000).Sub-additive or synergistic anti-angiogenic effect was observed when combined low dose Vinorelbine with hyperthermia in vitro.Low dose Vinorelbin has the ability of inhibiting migration and tube formation of HUVEC.Furthermore, it could induce apoptosis of HUVEC.We also observed that Vinorelbine suppressed the neovascularization of CAM in vivo. Conclusion The results of this experiment show that low dose of vinorelbine has anti-angiogenic effect both in vivo and in vitro, and there is additional effect when in combination with hyperthermia.