Inhibition Effect of Pancreatic Cancer PANC-1 Cells by shRNA Targeting on EGFR

ObjectiveTo explore the effect of EGFR gene on proliferation pancreatic cancer PANC-1cells. Methods EGFR gene sequence-specific shRNA expression vector was constructed and then transfected into pancreatic cancer PANC-1 cells with lipofectamine.The EGFR mRNA and protein expression was detected by RT-PCR and Western blot.The cell cycle distribution and apoptosis were detected by cell flow cytometry.The proliferation of cells was detected by clone formation assay. Results Sequence-specific shRNA targeting on EGFR gene can obviously repress the mRNA and protein expression of EGFR gene,and EGFR mRNA and protein inhibition rates was 72.1% and 67.6%,respectively.The cell cycle in G1 phase and S phase decreased(P＜0.05).The apoptosis of cells was increased(P＜0.05)and the colony-formation of cells was reduced(P＜0.05). Conclusion Sequence-specific shRNA targeting on EGFR gene can effectively inhibit proliferation and promote the apoptosis of pancreatic cancer cells.