Effects of Fuzheng Jiedu Granula on Nickel-induced DNA Damage in Rrats Renal Cells

ObjectiveTo study the effect of Fuzheng Jiedu Granula（FJG）on DNA damage in rats renal cells induced by Nickel sulfate(NiSO4). Methods Rats nephrotoxicity was induced by intraperitoneal injection of NiSO4［2.5 mg/(kg·d)］ and different dosages of FJG ［5,10,20 mg/(kg·d)］were administered by gavage. Cellar DNA single strand break(SSB) and total antioxidant capacity (TAC) of renal tissues were determined with single cell gel-electrophoresis (SCGE) assay and colorimetry,respetively. ResultsThe percentage of comet-like tail cells,length of tail,tail Olive moment,tail DNA percentage and ratio of tail/head were increased as well as TAC was increased significantly in FJG groups, compered with those in NiSO4 group（P<0.05, P<0.01). ConclusionBy inhibiting oxidative damage,FJG could play in antagonistic effect on DNA damage induced by nickel.